Figure 2.

PT recovers the decrease of filaggrin induced by IFN-γ and TNF-α. HaCaT cells were preincubated in the absence and presence of PT at the indicated concentrations for 1 h. The cells were then incubated with 10 ng/mL IFN-γ and TNF-α for 48 h. The harvested cells were lysed, and filaggrin, loricrin and involucrin were analyzed by western blotting. Densitometric data are expressed as a mean ± SD and are presented relative to the negative control, which was set at 1 (right panel) with statistical significance as *P < 0.05 and **P < 0.01 between the untreated and IFN-γ and TNF-α-treated group or between the IFN-γ and TNF-α-treated group and the PT-treated group.