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. 2019 Aug 6;16(8):1132–1141. doi: 10.7150/ijms.34386

Figure 5.

Figure 5

The effects of RPL15 ablation on cell proliferation and viability in various human cells. (A-B) RPE1 (A) and HCT116 (B) were transfected with NS siRNA or RPL15 siRNA and cell proliferation was determined by MTT assay at indicated times. Results represent means ± standard deviations of five independent experiments. (C) Cells transfected with NS siRNA or RPL15 siRNA for 48 h were labeled with 10 mM BrdU (1 h). The cells were fixed and immunostained with mouse α-BrdU. DNA was visualized by DAPI staining. Histograms represented the mean percentage of BrdU incorporation in indicated human cells depleted of RPL15. *P<0.05 and **P<0.01. (D) Cells were transfected with NS siRNA or RPL15 siRNA for 48 h. The cells were fixed and stained with Annexin V and PI. DNA was visualized by DAPI staining. Scale bars, 10 μm. (E) Histograms represented the mean percentage of Annexin V-positive cell in indicated human cells depleted of RPL15. ***P<0.001. (F) Immunoblotting to detect the effect of RPL15 depletion on cell cycle or apoptosis related proteins in indicated human cells. Cells were transfected with NS siRNA or RPL15 siRNA for 48 h. Whole cell lysates were immunoblotted with indicated antibodies.