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. 2019 Jul 20;15(9):1882–1891. doi: 10.7150/ijbs.31999

Figure 2.

Figure 2

IL-6 induced EMT through activation of STAT3. Cells were transfected with either STAT3 siRNA (siSTAT3) or negative control siRNA (NC siRNA) and activation of STAT3 was measured by western blot (A). Cells were incubated with IL-6 (50ng/ml) with or without AG 490 (50uM/L) for 48 hours and harvested for western blot of EMT markers (B) and JAK/STAT3 signaling molecules (D). Cells were transfected with siSTAT3 and EMT markers (C) and JAK/STAT3 signaling molecules (E) were measured by western blotting. Cells were treated with IL-6 with or without IL-6 antibody and EMT markers were measured (F). Blots were representatives of three experiments.