Figure 6.

Inhibiting STAT3 partly restores gefitinib sensitivity. (A to D) PC-9 cells were treated with gefitinib (50nmol/L) for 48 h. Cells were stained with annexin V-FITC and PI and subsequently analyzed using flow cytometry. (E) Quantification of gefitinib-induced apoptotic cells by annexin/PI double-staining. Apoptosis was evaluated by determining the percentage of annexin V-positive cells. The data are representative of three independent experiments and compared between IL-6 treated cells and gefitinib group, or between IL-6 plus an inhibitor co-treated cells and IL-6 group. *, p<0.05. **, p<0.01. #, p>0.05 not significant.