Figure 4.

CHL1 inhibits epithelial-mesenchymal transitions (EMT) and formation of F-actin and activation of Rho/Rac1 GTPases. (A) Cell morphology of CHL1-transfected and vector-transfected cells. (B) Western blot analysis was used to compare expression levels of epithelial and mesenchymal markers between empty vector- and CHL1-transfected cells. β-actin was used as loading control. (C) Stress fiber, lamellipodia and filopodia were formed in vector-transfected cells, but not in CHL1-transfected cells (magnification 400×). (D) Total and active forms of Rho-GTPases, including RhoA, Rac1, and Cdc42, were compared with CHL1-transfected and vector-transfected cells. GTP-bound (active) forms of RhoA, Rac1, and Cdc42 were pulled down and detected by western blot. Active forms of RhoA and Rac1 were much lower in CHL1-transfected SUNE1 cell lines than that in vector-transfected cell lines.