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. Author manuscript; available in PMC: 2019 Sep 13.
Published in final edited form as: Proteomics. 2018 Apr 16;18(11):e1700294. doi: 10.1002/pmic.201700294

Figure 3.

Figure 3.

Effect of type of fixation on protein yield and MS results from 1000 cells. A) Silver-stained representative gel from 1) unfixed cells (0.1 μg of protein); 2) 1000 GLU/PFA fixed cells; 3) 1000 PFA/DTBP fixed cells. B) Graphical representation of Spearman correlation between a representative replicate from the triplicate a) GLU/PFA or b) PFA/DTBP samples. The p-values <0.05 were considered statistically significant. Statistical analyses were done using StatPlus Pro. C) Venn diagram of identified proteins shared in the triplicate samples from a) 1000 GLU/PFA or b) 1000 PFA/DTBP fixed cells. D) Venn diagram showing the overlap of identified proteins between PFA/DTBP and GLU/PFA fixed samples. E) Average and SD of unique proteins, unique spectra, and unique peptides of samples from 1000 PFA/DTBP or GLU/PFA fixed cells. F) Comparison of unique proteins identified in 1000 PFA/DTBP fixed cells, 1000 or 1500 air fixed cells,[10] and 1500 PFA fixed cells extracted with two methods (A and B).[10] G) Comparison of unique peptides identified in 1000 PFA/DTBP fixed cells, 1000 or 1500 air fixed cells,[10] and 1500 PFA fixed cells extracted with two methods (A and B).[10]