Fig. 1.

Phenotypic characterization of MDSCs. PBMCs were isolated from heparinized blood of CAPS patients and healthy age-matched controls using Ficoll density gradient sedimentation. a MDSCs were identified by flow cytometry as neutrophilic cells which are CD66b^high, CD33^high, CD16^high, CXCR4^high, and HLA-DR^low, as shown representatively in the FSC-SSC (forward scatter-side scatter) dot blots and histograms. b Representative May-Grünwald-Giemsa-stained cytospins of conventional neutrophils (PMNs) and neutrophilic MDSCs are shown. c Mean fluorescence intensities of chemokine receptors CXCR4 and CCR2 in PMNs and neutrophilic MDSCs were determined by flow cytometry.