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Flow chart of the experiment. We seeded 1 × 106 wild-type mESCs for 1 day of culture (cell culture device A), 2 × 105 wild-type mESCs for 5 days of culture (cell culture device B), 1 × 106mRad9-/- mESCs for 1 day of culture (cell culture device C) and 4 × 105 mRad9 −/− mESCs for 5 days of culture (cell culture device D). The four cell culture devices were divided into two groups, with cell culture devices A and C in group 1 and cell culture devices B and D in group 2.
