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. 2019 Sep 1;30(19):2527–2534. doi: 10.1091/mbc.E19-03-0136

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© 2019 Shi, Wu, Miao, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 3: — Adenosine (NECA) regulates the expression of the stemness and EMT-associated genes through the A2aR pathway in vitro. Epithelial and mesenchymal marker expression was analyzed by immunoblotting (A) and immunofluorescence staining (B). Scale bar = 50 µm. (C) Immunoblotting detection of the stemness markers. The adenosine-induced increased stemness was confirmed by flow cytometry (D) and colony formation assay (E), but the specific antagonist of A2aR (SCH 58261) could reverse this effect. **, P < 0.01, ANOVA. All results are from three independent experiments.