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. 2019 Sep 10;7:e7195. doi: 10.7717/peerj.7195

Table 2. PCR program for SNPs.

PCR steps Temperature (°C) Duration of steps No. of cycles
Initial duration 92 5 min 30–35
Denaturation 94 30 sec
Annealing rs35958982 58.4 1 min
rs3780135 56.6
rs12430881 58.8
Extension 72 1 min
Final extension 72 5 min