Table 2. PCR program for SNPs.
PCR steps | Temperature (°C) | Duration of steps | No. of cycles |
---|---|---|---|
Initial duration | 92 | 5 min | 30–35 |
Denaturation | 94 | 30 sec | |
Annealing rs35958982 | 58.4 | 1 min | |
rs3780135 | 56.6 | ||
rs12430881 | 58.8 | ||
Extension | 72 | 1 min | |
Final extension | 72 | 5 min |