TABLE 1:

Combined results from the primary and secondary RNAi screens.

				2o screen results
1o screen hits	Gene symbol	siRNA IDs		% actin tails	% confluency
Caveolin 1	CAV1	s2447	s2448	+	+
Caveolin 2	CAV2	s2451	s2449	+	+
Pacsin2	PACSIN2	s229822	s22215	+	+
Rac1	RAC1	s11712	s11713	+	+
Flotillin 1	FLOT1	s19915	s19914	+	+
Beta-arrestin 1	ARRB1	s1622	s1623	+	+
Tspan30	CD63	s2699	s2700	+	+
E-cadherin	CDH1	s2768	s2769	+	+
p120 catenin	CTNND1	s3725	s3727	+	+
Gamma-catenin	JUP	s7668	s7666	+	+
Desmocollin-2	DSC2	s4311	s4310	+	+
Ephrin-A2	EFNA2	s194390	s4500	+	+
Ephrin-A3	EFNA3	s4502	s4503	+	+
Ephrin type B receptor 2	EPHB2	s4742	s4741	+	+
Connexin 30.3	GJB4	s43186	s43185	+	+
Connexin 46	GJA3	s5762	s5760	+	+
ZO-1	TJP1	s14155	s14157	+	+
Claudin-6	CLDN6	s17309	s17310	+	+
Fes	FES	s5114	s5113	+	+
Tuba	DNMBP	s23437	s23439	+	+
srGAP2	SRGAP2	s23692	s23691	+	+
CHMP3a	VPS24	s28474	s28473	+	+
Alpha-catenin 2	CTNNA2	s3719	s3721	+	−
CapZB	CAPZB	s2402	s2401	−	+
Cdc42	CDC42	s2767	s2766	+	−
hVps28	VPS28	s27578	s27579	+	−
JAM-C	JAM3	s38127	s38129	+	−
Nectin 2	PVRL2	s11607	s11606	−	−
Talin 1	TLN1	s14187	s14185	+	−
PSTPIP1	PSTPIP1	s17257	s17255	+	−

Examples of genes not identified in 1o screen
Clathrin heavy chain	CLTC
AP2-u2	AP2M1
Ephrin-A4	EFNA4
Ezrin	EZR
Occludin	OCLN

^aCHMP3 knockdown caused an increase in focus size; the other 1^o screen hits showed a decrease in focus size. For % confluency: +, no decrease; −, reduced confluency (p < 0.05) for at least one siRNA/gene. For % actin tails: +, no decrease; −, reduced tail frequency (p < 0.05) for at least one siRNA/gene. See also Supplemental Figures S1 and S2.