TABLE 1:

Inhibition of glycerol-3-phosphate dehydrogenase activity by As(III), MAs(III), and As–biotin.

	Activity (%)
Inhibitor	gpd1Δgpd2Δ	mtq2Δ
None	100	100
As(III) 10 μM	81 ± 5.3	NT
As(III) 100 μM	51 ± 3.4	65 ± 3.6
As(III) 1 mM	15 ± 1.2	9.0 ± 1.0
MAs(III) 1 μM	55 ± 3.7	NT
MAs(III) 5 μM	23 ± 1.1	NT
MAs(III) 10 μM	4.0 ± 0.3	NT
As–biotin 1 μM	2.7 ± 0.3	NT

Glycerol-3-phosphate dehydrogenase activity was measured in a gpd1Δ gpd2Δ strain (DL4285) or an mtq2Δ strain (DL4313) expressing Gpd1-TAP borne on a plasmid (p3467) and under the control of the inducible GAL1 promoter. NT: not tested. Values are the mean and SD from three independent experiments.