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. 2019 Aug 1;30(17):2254–2267. doi: 10.1091/mbc.E19-01-0009

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© 2019 Faust, Balabiyev, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Mixing macrophages expressing eGFP-LifeAct and mRFP-LifeAct shows asymmetric actin integration. (A) Macrophage fusion in a mixed population of eGFP/mRFP-LifeAct macrophages. Two mononuclear cells expressing eGFP-LifeAct and mRFP-LifeAct undergoing fusion are outlined in the image taken at 5:57 min. A site of eGFP- and mRFP-LifeAct integration is indicated by white arrowheads at 6:14 and 6:48 min. (B) Split-channel view of the fusion event shown in A. The boxes outlined in white dashed lines in the images taken at 6:14–6:48 min correspond to the areas where integration of mRFP-LifeAct into the eGFP-LifeAct-containing cells is observed. The enlarged images of the same areas are shown as insets. The scale bars are 5 μm. See also Supplemental Videos S9 and S10.