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. 2019 Aug 1;30(17):2141–2154. doi: 10.1091/mbc.E19-02-0094

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© 2019 Goodrum et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — Fzo1 depletion triggered by aging and vacuole impairment is proteasome and Doa1-dependent. (A) Whole cell extracts from yeast expressing Fzo1-HA grown in the absence or presence of concanamycin A (ConcA) or proteasome inhibitor MG-132 for 4 h were analyzed by Western blot with anti-HA and anti-Pgk1 antibodies. (B, C) Whole cell extracts from wild-type (WT), atg5Δ, and pep4Δ (B) or WT and doa1Δ (C) yeast expressing Fzo1-HA grown in the absence or presence of concanamycin A for 4 h were analyzed as in A. (D) Whole cell extracts from young (Y) and old (O) doa1Δ yeast expressing Fzo1-HA protein were analyzed as in A. Age ranges (n = 30 cells): Y = 0–3 divisions; O = 6–10 divisions.