TABLE 1:
Yeast strains and oligos used in this study.
| Strain | Parent and genotype |
|---|---|
| BY4741 | MATa his3Δ1 leu2Δ0 ura3Δ0 met15Δ0 |
| BY4742 | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 |
| BY4743 | MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 met15Δ0/+lys2Δ0/+ |
| AHY1443 | BY4741 TOM70-yeGFP:KanMX |
| AHY3417 | BY4741 TOM70-yeGFP:KanMX doa1Δ::URA3 |
| AHY1919 | BY4743 TOM70-yeGFP:KanMX/+ |
| AHY3324 | BY4741 FZO1-3xHA:KanMX |
| AHY3720 | BY4741 FZO1-3xHA:KanMX pdr5Δ::URA3 |
| AHY3716 | BY4741 FZO1-3xHA:KanMX atg5Δ::URA3 |
| AHY3714 | BY4741 FZO1-3xHA:KanMX pep4Δ::URA3 |
| AHY8285 | BY4741 FZO1-3xHA:KanMX pep4Δ::URA3 prb1Δ::LEU2 |
| AHY3619 | BY4741 FZO1-3xHA:KanMX doa1Δ::URA3 |
| AHY4758 | BY4741 FZO1-3xHA:KanMX mdm30Δ::HygMX |
| AHY3450 | BY4741 fzo1-PY (LPHY→AAAA)-3xHA:KanMX |
| AHY3453 | BY4741 fzo1-PY (LPHY→AAAA)-3xHA:KanMX mdm30Δ::HygMX |
| AHY7089 | BY4741 FZO1-3HA:KanMX ChrI nt199459::GPDp-URA3 |
| AHY7091 | BY4741 FZO1-3HA:KanMX ChrI nt199459::GPDp-AVT1-URA3 |
| AHY8286 | BY4741 FZO1-3xHA:KanMX gpr1Δ::LEU2 |
| AHY8288 | BY4741 FZO1-3xHA:KanMX gpa2Δ::LEU2 |
| AHY8290 | BY4741 FZO1-3xHA:KanMX ssy1Δ::LEU2 |
| AHY4605 | MATα his3Δ1 leu2Δ0 ura3Δ0 fzo1-PY (LPHY→AAAA) TOM70-yeGFP:KanMX mdm30Δ::HygMX |
| R1158 | BY4741 URA3::CMV-tTA |
| AHY3850 | R1158 FZO1-3xHA:His3MX |
| AHY3852 | R1158 RSP5p::kanR-tet07-TATA FZO1-3xHA:His3MX |
| AHY3910 | R1158 FZO1-3xHA:His3MX mdm30Δ::HygMX |
| AHY3911 | R1158 RSPp5::kanR-tet07-TATA FZO1-3xHA:His3MX mdm30Δ::HygMX |
| AHY1421 | R1158 TOM70-yeGFP:spHIS5MX |
| AHY1422 | R1158 RSP5p::kanR-tet07-TATA TOM70-yeGFP:spHIS5MX |
| AHY1424 | R1158 TOM70-yeGFP:spHIS5MX mdm30Δ::HygMX |
| AHY1430 | R1158 RSP5p::kanR-tet07-TATA TOM70-yeGFP:spHIS5MX mdm30Δ::HygMX |
| UCC8773 | MATa his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2 |
| UCC895 | MATa his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2 TOM70-yeGFP:KanMX |
| AHY490 | MATα his3Δ1 leu2Δ0 ura3Δ0 trp1Δ63 hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2 TOM70-mCherry:KanMX |
| AHY923 | MATa his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2 TOM70-yeGFP:KanMX dnm1Δ::URA3 |
| AHY937 | MATα his3Δ1 leu2Δ0 ura3Δ0 trp1Δ63 hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2 TOM70-mCherry:KanMX dnm1Δ::URA3 |
| AHY546 | MATa/α his3Δ1/hisΔ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/+ trp1Δ63/+hoΔ::SCW11p-Cre-EBD78-NatMX/hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX/loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2/loxP-UBC9-loxP-LEU2 |
| AHY951 | MATa/α his3Δ1/hisΔ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/+ trp1Δ63/+hoΔ::SCW11p-Cre-EBD78-NatMX/hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX/loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2/loxP-UBC9-loxP-LEU2 TOM70-yeGFP-KanMX/+ VPH1-mCherry-KanMX/+ |
| AHY995 | MATa/α his3Δ1/hisΔ1 leu2Δ0/leu2Δ0 ura3Δ0/ura3Δ0 lys2Δ0/+ trp1Δ63/+hoΔ::SCW11p-Cre-EBD78-NatMX/hoΔ::SCW11p-Cre-EBD78-NatMX loxP-CDC20-Intron-loxP-HphMX/loxP-CDC20-Intron-loxP-HphMX loxP-UBC9-loxP-LEU2/loxP-UBC9-loxP-LEU2 TOM70-yeGFP:KanMX/+ VPH1-mCherry:KanMX/+ dnm1Δ::URA3/ dnm1Δ::URA3 |
| JSY2519 | MATa ura3-52 his3Δ200 trp1Δ63 ura3Δ met15Δ MGM1:3xHA (W303) |
| Oligo name | Oligo sequence |
|---|---|
| FZO1 pFA6a tag F | TCAAAAATTGATGGTGGAAGAAATAAATTTAGACATCGATCGGATCCCCGGGTTAATTA |
| FZO1 pFA6a tag R | TATATTGATTTGAAAAGACCTCATATATTTACAAGAATATGAATTCGAGCTCGTTTAAAC |
| FZO1 tag check | CCCGTGCCTTACCAATGAATCTTTCC |
| HA tag check R | ACGTCATATGGATAGGATCC |
| TOM70 pKT tag F | TCAAGAAACTTTAGCTAAATTACGCGAACAGGGTTTAATGGGTGACGGTGCTGGTTTA |
| TOM70 pKT tag R | TTTGTCTTCTCCTAAAAGTTTTTAAGTTTATGTTTACTGTTCGATGAATTCGAGCTCG |
| VPH1 pKT tag F | GGAAGTCGCTGTTGCTAGTGCAAGCTCTTCCGCTTCAAGCGGTGACGGTGCTGGTTTA |
| VPH1 pKT tag R | AGTACTTAAATGTTTCGCTTTTTTTAAAAGTCCTCAAAATTCGATGAATTCGAGCTCG |
| PDR5 KO F | AAGTTTTCGTATCCGCTCGTTCGAAAGACTTTAGACAAAAAGATTGTACTGAGAGTGCAC |
| PDR5 KO R | TCTTGGTAAGTTTCTTTTCTTAACCAAATTCAAAATTCTACTGTGCGGTATTTCACACCG |
| PDR5 KO check | CGGAACTCTTCTACGCCGTGGTACGATATC |
| URA check | ACCTAATGCTTCAACTAACTCCAG |
| PEP4 KO F | ATTTAATCCAAATAAAATTCAAACAAAAACCAAAACTAACGATTGTACTGAGAGTGCAC |
| PEP4 KO R | GGCAGAAAAGGATAGGGCGGAGAAGTAAGAAAAGTTTAGCCTGTGCGGTATTTCACACG |
| PEP4 KO check | GCCTAGTGACCTAGTATTTAATCC |
| ATG5 KO F | GGTTCTAGAAGAACGGAGATAGGAAACCTATGATGTAAGTGATTGTACTGAGAGTGCAC |
| ATG5 KO R | GATATTTGAATGACACTTTTAAATGCGTATCACACCGTATAACAGCTCCTGTGCGGTATT |
| ATG5 KO check | CACTAAATAATGAAGTAGCATGCTCAG |
| DOA1 KO F | TCATGTGTGATAGTAAGGTGTAGAGCAGCAGATTTGGAGTAGATTGTACTGAGAGTGCAC |
| DOA1 KO R | ATCTAGACATTATGTGTTTTATATGATTGCTGTAAAAGTACTGTGCGGTATTTCACACCG |
| DOA1 KO check | ATGCAACTCCAGTAGCTTGATATATTCGGC |
| MDM30 KO F | ACAATTTTTCGGTATTAGTTACTAAAAGGCTCACATATACAGATTGTACTGAGAGTGCACC |
| MDM30 KO R | TGTGTCAGGATGCTACTTTTGGAAACCTCCTTAAATATGACTGTGCGGTATTTCACACCG |
| MDM30 KO Check | GTTTATGTTACATAATAAGTGAAAG |
| DNM1 KO F | TTAAGTAGCTACCAGCGAATCTAAATACGACGGATAAAGAGATTGTACTGAGAGTGCACC |
| DNM1 KO R | TGTTGAAGTAAGATCAAAAATGAGATGAATTATGCAATTACTGTGCGGTATTTCACACCG |
| DNM1 KO Check | GTGGGAACAATAAATAAGAGGGTAGG |
| Hyg Check | TATGCTCCGCATTGGTCTTG |
| URA3 mid R | CCACGGTTCTATACTGTTGAC |
| Ura3 mid F | GCTACATATAAGGAACGTGCTG |
| FZO1 PY Adapt 1 | CGGTGTGAAATACCGCACAGGCTGCTGCTGCTCATAATGAAAATGACAATGAAGATCATG |
| FZO1 PY Adapt 2 | GGTGCACTCTCAGTACAATCTAATCGATGTCTAAATTTATTTCTTCCACC |
| FZO1 PY Mutamer | GTTTCCAAAAACGGAGATGAAGCTGCTGCTGCTCATAATGAAAATGACAATG |
| pRS306 RS2 R | CTGTGCGGTATTTCACACCG |
| pRS306 RS1 F | ATTGTACTGAGAGTGCACC |
| pRS306 RS1 R | GGTGCACTCTCAGTACAAT |
| PRB1 KO F | CAATAAAAAAACAAACTAAACCTAATTCTAACAAGCAAAGCTGTGCGGTATTTCACACCG |
| PRB1 KO R | AAGAAAAAAAAAAGCAGCTGAAATTTTTCTAAATGAAGAAAGATTGTACTGAGAGTGCAC |
| PRB1 KO Check | AGATGATGTTATTGTTGCGG |
| SSY1 KO F | GTTATAAACTGCTTAAAAATAGGGAAGTTCCTTGAGGAATAGATTGTACTGAGAGTGCAC |
| SSY1 KO R | AATACTAACAATAATAATACTAATAATAGTACATATAACCCTGTGCGGTATTTCACACCG |
| SSY1 KO Check | TTAATCCTGTTACTCCTGTGCTAGATGGGG |
| GPA2 KO F | TTGTTACAGCACAAATCACGCGTATTTTCAAGCAAATATCAGATTGTACTGAGAGTGCAC |
| GPA2 KO R | AGAAGAGGCATGCAGTTTTGTCTCTGTTTTAGCTGTGCATCTGTGCGGTATTTCACACCG |
| GPA2 KO Check | GATATACCATATATTACGATCCCTGCGCCC |
| GPR1 KO F | ATCCGAAGTGTGACGAATAAAGCAAACTCTCCAACTCAAAAGATTGTACTGAGAGTGCAC |
| GPR1 KO R | TTCCTTACTTTCCATTTTCAAACATCGCGATACAAAAACTCTGTGCGGTATTTCACACCG |
| GPR1 KO Check | CACAAGCTGACCCTAATTACCAAGGGACCT |