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. 2019 Aug 1;30(17):2296–2308. doi: 10.1091/mbc.E19-01-0069

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© 2019 Hellerschmied, Serebrenik, et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.

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FIGURE 1: — Degradation profiles of Golgi-localized model QC substrates. (A) Domain outline of Golgi QC substrates targeted to the cis- (MAN2A1-EGFP-HT2) and trans- (ST6GAL1- and B4GT-EGFP-HT2) Golgi. (B) Flow cytometry analysis quantifying the levels of MAN2A1-EGFP-HT2 in HEK293 cells on the indicated treatments for 6 h (EPX, epoxomicin; BafA1, bafilomycin A). CHX was added 1 h prior to the indicated treatments. (C) Same analysis as in B for HEK293 cells expressing B4GT-EGFP-HT2 or ST6GAL1-EGFP-HT2 (n = 2, data represent mean ± SEM). (D) Flow cytometry analysis quantifying the levels of B4GT-EGFP-HT2 in HeLa cells on the indicated treatments for 6 h in the absence (left) or presence (right) of CHX (n = 3, data represent mean ± SEM, results of t test are shown).