2.

EZH2 depletion has little impact on MCL cells. (A, B) Z138 cells were introduced with CRISPR/Cas9 system targeting EZH2. After 4 weeks of puromycin selection, EZH1 and EZH2 expression were determined on mRNA and protein levels respectively. (C-E) Upon EZH2 KD, cell proliferation was determined by MTS assay; cell cycle progression and apoptosis were determined by flow cytometry. (F-J) Z138 cells were treated with EPZ005687 and determined with EZH1/EZH2 expression, cell proliferation, cell cycle progression, and apoptosis. Data are represented as the mean ± SD. All experiments were repeated at least three times. ** P < 0.01.