3.

KCTD12 downregulation promotes A375 cells’ proliferative and metastasis-related capacities. (A) KCTD12 knockdown via siRNA in A375 cells was confirmed by RT-qPCR (left panel) and Western blot (middle and right panels). (B) Proliferative capacity and (C) colony formation capacity (8 days) of A375 cells were enhanced after KCTD12 knockdown. (D) Gelatin adhesion capacity and (E) HUVEC adhesion capacity were increased after KCTD12 knockdown in A375 cells (1 h). Scale bars: 200 μm. (F) Transmigration capacity and (G) invasion capacity of A375 cells were strengthened after KCTD12 knockdown (24 h). Scale bars: 50 μm. Data are mean ± SD (n= 3 - 5); *, P < 0.05; **, P < 0.01, determined by one-tailed unpaired t-test.