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. 2019 Aug;16(3):498–513. doi: 10.20892/j.issn.2095-3941.2019.0073

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Copyright 2019 Cancer Biology & Medicine

This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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KCTD12 knockdown endows B16F10 and A375 cells the CSC-like traits. (A) KCTD12 knockdown decreased B16F10 cells’ melanogenesis and the corresponding tyrosinase activity. (B) The upregulation of melanoma CSC marker CD271 was confirmed by RT-qPCR (left panel) and Western blot (middle and right panels) after KCTD12 knockdown in B16F10. (C) Expression of genes associated with melanogenesis, glycolysis, invasion, EMT, differentiation and CSC after KCTD12 knockdown in B16F10 were detected by RT-qPCR. (D) The upregulation of melanoma CSC marker CD271 was confirmed by RT-qPCR (left panel) and Western blot (middle and right panels) upon KCTD12 knockdown in A375. (E) Genes related to melanogenesis, CSC, adhesion, and invasion were detected after KCTD12 knockdown in A375 by RT-qPCR and FACS. Data are mean ± SD (n = 3 - 5); *, P < 0.05; **, P < 0.01, determined by one-tailed unpaired t-test.