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. 2019 Aug;16(3):498–513. doi: 10.20892/j.issn.2095-3941.2019.0073

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Copyright 2019 Cancer Biology & Medicine

This work is licensed under a Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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(A) The photographs of spheroids formed by A375 cells and A375-KCTD12-KO cells at the eighth day. (B) The quantification of spheroid diameter from the first day to the eighth day. Data are mean ± s.d. (<italic>n</italic> = 3 - 5); *, <italic>P</italic> < 0.05; **, <italic>P</italic> < 0.01, determined by one-tailed unpaired <italic>t</italic>-test. — The elevated spheroid formation ability was confirmed by spheroid formation assay after KCTD12 knockout in A375 cells.