S6.

Inhibition of PKCα-ERK1/2-Fra-1 signaling abolished the heat shock-induced malignant effects. (A) Sphere-forming assay of CT-26 cells treated with or without HS and with a panel of different inhibitors, 10058-F4 (500 nM), Gö6976 (500 nM), U0126 (5 μM), TG101348 (500 nM), XAV939 (500 nM), Ikk-16 (500 nM) and Dasatinib (500 nM). Representative whole view images of spheres are shown. (B) Quantification of the spheres by Gel count machine and MTT assay. *P < 0.05, ** P < 0.01. n = 3.