2.

Effects of CSN6 expression on proliferation in GC cells. (A) CCK-8 assay was performed in MKN-45 cells and MGC-803 cells after transfection of pCDNA3.1-CSN6/pCDNA3.1 empty vector expression plasmids and control siRNA/CSN6 siRNA for 24, 48, 72 and 96h. (B) The effects of CSN6 on the colony formation ability of CSN6-knockdown and CSN6-overexpression in MKN-45 and MGC-803 cell lines. (C) CSN6 expression promotes cell proliferation of EdU incorporation. MKN-45 and MGC-803 cells were transfected with CSN6 and fixed for anti-EdU staining (original magnification, 100×). EdU-positive cells were counted and are presented as a bar graph. (D) The percentage of G1 and S population cells was measured by flow cytometry after CSN6 overexpression in MKN-45 and MGC-803 cells. After the knockdown of CSN6 in GC cells, the percentage of cells at G1 and S stage was calculated using ModFit LT 3.0 software. The data represent the mean ± SD (*P ≤ 0.05, **P ≤ 0.01). All experiments were performed in triplicate.