6.

Involvement of CSN6 and p16 in tumorigenesis. (A-C) Overexpression of CSN6 promoted tumorigenicity. CSN6-overexpressing MKN-45 or MGC-803 stable transfectants were subcutaneously injected into the right side of the dorsal flanks of eight nude mice. Simultaneously, their corresponding control cells were injected into the left side of the same mice. The tumor volumes of the individual mice were measured every 5 days. Tumors were collected at the end of the assay, and the tumor weight of each group was measured. The data represent the mean±SD (*P ≤ 0.05, **P ≤ 0.01). (D) CSN6 expression in xenograft mouse models regulated protein levels of p16. MKN-45 or MGC-803 cells with CSN6 overexpression or control vector were subcutaneously injected into the nude mice. The indicated tumor samples were isolated and analyzed by immunoblotting with the indicated antibodies. (E) Overexpression of CSN6 diminished p16 expression in tumors. Tumors were collected and representative tumor sections are shown (original magnification, 400×). The statistical graph indicates the proportion of positive cells (**P ≤ 0.01).