Fig 6. Bypassing cell cycle exit disrupts chromatin dynamics at ecdysone target genes and alters their expression.

(A) Scatterplot of ecdysone-responsive genes in 44-h wings under conditions that bypass cell cycle exit versus normal exit. Genes with significant changes in expression are labeled in red. (B, C) Chromatin regions of Blimp-1, Hr3, E74, and E75 fail to close or open at 44 h when cell cycle exit is compromised. (D) Blimp-1 antibody staining of wings at 36 h and 40–42-h wings with normal cell cycle exit (Ctr) or bypassed cell cycle exit in the posterior (using engrailed-Gal4/Gal80^TS). Compromising cell cycle exit delays the activation of Blimp-1 in a compartment-autonomous manner. (E) Peaks that fail to open at 44 h from cuticle-development genes harbor high-scoring Blimp-1 binding sites. The underlying data in Fig 6A can be found within S6 Data. Cda5, Chitin deacetylase-like 5; Cpr, Cuticular protein; Ctr, control; E2F, E2F transcription factor; Hr3, Hormone receptor 3; Gal80^TS, temperature-sensitive Gal80; RPKM, reads per kilobase of transcript, per million mapped reads; TEM, transmission electron microscopy; wt, wild type.