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Up- regulation of miR- 218 promotes osteosarcoma Saos- 2 cell apoptosis.A: Cell count of adhering living Saos- 2 cells transfected with miR-218 mimics or anti-miR-218 mimics and control Saos-2 cells, measured by CCK-8 at 24, 36 and 48 h; B: Morphological observation (phase- contrast microscopy, upper panel) and fluorescence microscopy (lower panel) of apoptotic cells 24 h after miR- 218 transfection with annexin V- FITC staining (original magnification: × 200, scale bar=50 μm); C: Flow cytometric analysis of apoptosis of Saos-2 cells and control cells 24 h after miR-218 transfection; D: Comparison of early, late and total apoptosis rates of Saos-2 cells between miR-218 transfected and non-transfected (control) groups; E, F: Western blotting and quantitative analysis of expression level of C-PARP protein relative to the control level in Saos-2 cells 24 h after transfection with miR-218 mimics or anti-miR-218 mimics.β-actin was used as a loading control.The data are presented as Mean±SD from 3 independent experiments (^*Ρ < 0.05).