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. 2018 May 20;38(5):505–510. doi: 10.3969/j.issn.1673-4254.2018.05.01

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MiR-218 directly targets BMI-1 in osteosarcoma Saos-2 cells.A: Predicted miR-218 target sequences in 3'UTR of BMI-1 mRNA and mutant BMI-1 mRNA containing 8 mutated nucleotides (BMI-1 mut); B: qRT-PCR analysis of BMI-1 expression in Saos- 2 cells transfected with miR- 218 mimics or anti- miR-218 mimics compared with negative control cells (*Ρ < 0.05); C, D: Western blotting and quantitative analysis of the expression level of BMI-1 relative to the control level in the transfected cells; E: Luciferase activity in Saos-2 cells co-transfected with miR-218 mimics and luciferase reporters containing either predicted miRNA target site in BMI-1 3'UTR or its corresponding mutant form (*Ρ < 0.05).