1.
引物序列
Primer sequence
| Genes | Forward prime (5'-3') | Reverse prime (5'-3') |
| TGF-β1:Transforming growth factor β1; α-SMA:α-smooth muscle actin; PPARγ:Peroxisome proliferator activated receptorγ; FAS:Fatty acid synthase; ACCα:Acetyl-CoA carboxylaseα; SREBP-1c:Sterol regulatory element binding transcription factor 1; PPARα:Peroxisome proliferator activated receptor α; Scd-1:Stearoyl-Coenzyme A desaturase 1; NOX4:NADPH oxidase 4; SOD1:Superoxide dismutase 1; HO-1:Heme oxygenase-1; Nrf2:Nuclear factor, erythroid derived 2; GPX4:Glutathione peroxidase 4. | ||
| TGF-β1 | TGTGTTGGTTGTAGAGGGCAAGGA | TTTGGAGCCTGGACACACAGTACA |
| α-SMA | TCGGATACTTCAGCGTCAGGA | GTCCCAGACATCAGGGAGTAA |
| Collagen-Ⅰ | GCAGGAGGTTTCGGCTAAGT | GCAACAAAGTCCGCGTATCC |
| PPARγ | ATTCTGGCCCACCAACTTCGG | TGGAAGCCTGATGCTTTATCCCCA |
| FAS | CTGCGGAAACTTCAGGAAATG | GGTTCGGAATGCTATCCAGG |
| ACCα | GGCCAGTGCTATGCTGAGAT | AGGGTCAAGTGCTGCTCCA |
| SREBP-1c | CACTTCTGGAGACATCGCAAAC | ATGGTAGACAACAGCCGCATC |
| PPARα | TATTCGGCTGAAGCTGGTGTAC | CTGGCATTTGTTCCGGTTCT |
| Scd-1 | TTCTTGCGATACACTCTGGTGC | CGGGATTGAATGTTCTTGTCGT |
| NOX4 | TGCCTGCTCATTTGGCTGT | CCGGCACATAGGTAAAAGGATG |
| SOD1 | GGACCTCATTTTAATCCTCACTCTA | CACACGATCTTCAATGGACACA |
| HO-1 | AGACCGCCTTCCTGCTCAACAT | TCTGACGAAGTGACGCCATCTGT |
| Nrf2 | CTTTAGTCAGCGACAGAAGGAC | AGGCATCTTGTTTGGGAATGTG |
| GPX4 | CCACGCAGCCGTTCTTAT | GAGGCAGGAGCCAGGAAGT |
| β-actin | GTCCACCCCGGGGAAGGTGA | AGGCCTCAGACCTGGGCCATT |