. 2017 Dec 20;37(12):1648–1653. [Article in Chinese] doi: 10.3969/j.issn.1673-4254.2017.12.15

2.

粪便q-PCR艰难梭菌tpi、毒素基因（tcdA/tcdB）所用引物

Primers for q-PCR of CD tpi and toxin gene (tcdA/tcdB) in stool specimens

Gene	Primer	Base sequence 5'→3'	Product (bp)
tpi
	tpi-qF1 tpi-qR1 tpi-pro1	GTCATTAGCATCTTCAGCAG TAGAACAAAGAGAAGCTGG TACCAGTTCCAATAGCCCAGAT	164
tcdA
	tcdA-qF tcdA-qR tcdA-pro	GCTTTCGCTTTAGGCAGTGT GAGTTTTCTGCGGTAGCTGA CCAACACCTTAACCCAGCCATAGAG	206
tcdB
	tcdB-qF tcdB-qR tcdB-pro	GCACCTGCTAATACTGTAAATG GTCTCAATTGTATATGTTTCTCC TACGGACAAGCAGTTGAATATAGTGG	113