1.
总RNA的定量与质检结果
Quantity and quality control of the total RNA extracted from the cells
| Sample ID | A260/280 Ratio | A260/230 Ratio | Conc (ng/pL) | Volume (pL) | Quantity (ng) | QC result (pass or fail) |
| The acceptable A260/280 ratio for pure total RNA is between 1.8 and 2.1. The acceptable A260/230 ratio for pure total RNA should be more than 1.8. | ||||||
| MCF10A-1 | 1.99 | 2.36 | 837.1 | 80 | 66968 | Pass |
| MCF10A-2 | 1.98 | 2.34 | 769.49 | 80 | 61559.2 | Pass |
| MCF10A-3 | 2.03 | 2.39 | 864.13 | 80 | 69130.4 | Pass |
| MCF7-1 | 2.05 | 2.29 | 893.75 | 60 | 53625 | Pass |
| MCF7-2 | 1.99 | 2.41 | 796.24 | 60 | 47774.4 | Pass |
| MCF7-3 | 2.01 | 2.4 | 918.99 | 60 | 55139.4 | Pass |