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RT-PCR中内参及目的基因引物序列
Primers and probes used for real-time PCR
| Gene name | Primer and Probe Sequence | Product Fragment | Gene bank accession number |
| GADPH | 96 bp | NM_017008.3 | |
| 5' CAACGGGAAACCCATCACCA 3' 5' ACGCCAGTAGACTCCACGACAT 3' |
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| TRPC6 | 162 bp | NM_053559.1 | |
| 5' CGGCAGACAGTTCTTCGTGAG 3' 5' ACATTCTTCTAGCATCTTCCGCAC 3' |
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| VEGF | 147 bp | NM_022628.1 | |
| 5' TTCGTCCAACTTCTGGGCTCTT 3' 5' CTCCTCTTCCTTCTCTTTCTCCC 3' |
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| Nephrin | 190 bp | NM_022628.1 | |
| 5' TGTGTACCTGGGGTCTTGGA 3' 5' AGGACTGGTTCGTCTTGTCG 3' |
