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Exposed or buried configurations of epitopes in the S protein globule. (A) Native SDS-PAGE. Unboiled samples were subjected to Western blot analysis. (B) Denaturing and reprobing. PVDF membranes from panel A were soaked in stripping buffer for 5 min to denature the bound S protein and subjected to detection with the same antibodies a second time. For both panels, Western blot analysis was performed using the indicated antibodies.
