FIG 4.

Analysis of dual mutants. (A) Schematic of the Bi-NLuc-SG positive and negative strands. On the negative strand, red indicates the mutated terminal and internal site. (B and C) Measurement of Nano (red) and firefly (blue) luciferase activities of the mutants. ΔGDD, replication-deficient NS5B mutant Bars for luciferase activity represent the RLU without normalization (means ± the SD) of three biological replicates, each measured in technical duplicates. For a description of the mutations, refer to the legend for Fig. 3.