FIG 4.

Contribution of the alternative pathway of complement to Chandipura virus neutralization is limited. (A) Factor B reconstitution of the depleted serum was tested against NHS, with the depleted serum serving as the control using the standard hemolytic assay. (B) One hundred PFU of CHPV was incubated with either factor B-depleted (fB dep.) or -reconstituted serum (fB dep.+fB), and the CHPV+PBS or the CHPV+NHS served as the controls. Significance of the tests and NHS control was compared against the virus-only sample by taking the values from six independent experiments, with ** denoting a P value of ≤0.0001.