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. 2019 Sep 12;93(19):e00307-19. doi: 10.1128/JVI.00307-19

FIG 2.

FIG 2

Hyperphosphorylation of p-STAT1 in IFN-γ-treated STAT1 R274W T cells. Splenocytes from 6- to 8-week-old STAT1 R274W and WT littermate control mice were treated with vehicle (medium) or 100 ng/ml IFN-γ for 15 min followed by flow cytometric analysis of p-STAT1 expression levels in CD8+ and CD4+ T cells, B cells, and NK cells. (A) Representative flow cytometry histograms of p-STAT1 in CD8+ T cells, CD4+ T cells, B cells, and NK cells with and without treatment with IFN-γ. (B through E) Percent p-STAT1+, number of p-STAT1+, and MFI of p-STAT1 in CD8+ T cells (B), CD4+ T cells (C), B220+ B cells (D), and NK1.1+ NK cells (E). All data represent the mean of n = 5 to 9 samples per genotype from three independent experiments and were analyzed by unpaired t test (****, P < 0.0001; ***, P < 0.0005; **, P < 0.005; *, P < 0.05).