FIG 2.

Hyperphosphorylation of p-STAT1 in IFN-γ-treated STAT1 R274W T cells. Splenocytes from 6- to 8-week-old STAT1 R274W and WT littermate control mice were treated with vehicle (medium) or 100 ng/ml IFN-γ for 15 min followed by flow cytometric analysis of p-STAT1 expression levels in CD8⁺ and CD4⁺ T cells, B cells, and NK cells. (A) Representative flow cytometry histograms of p-STAT1 in CD8⁺ T cells, CD4⁺ T cells, B cells, and NK cells with and without treatment with IFN-γ. (B through E) Percent p-STAT1⁺, number of p-STAT1⁺, and MFI of p-STAT1 in CD8⁺ T cells (B), CD4⁺ T cells (C), B220⁺ B cells (D), and NK1.1⁺ NK cells (E). All data represent the mean of n = 5 to 9 samples per genotype from three independent experiments and were analyzed by unpaired t test (****, P < 0.0001; ***, P < 0.0005; **, P < 0.005; *, P < 0.05).