FIG 5.

Virus-specific antibody responses and flow cytometric quantitation of splenic lymphocyte subsets in γHV68-infected WT and STAT1 R274W mice. At ages of 5 to 6 weeks, WT and STAT1 R274W mice were inoculated intraperitoneally with 10⁶ PFU of γHV68 or PBS control (mock, day 0). (A and B) Serum was harvested on day 14 after infection. Virus-specific IgM (A) and IgG (B) levels were measured by ELISA. Data represent the mean of n = 16 biological replicates pooled from two independent experiments. P > 0.1 by unpaired t test. (C) Spleen weight prior to isolation of leukocytes. (D to G) Mice were euthanized and the spleens harvested at days 0, 4, 8, and 14 after infection for flow cytometric analysis. Numbers of CD45⁺ leukocytes (D), CD19⁺ B cells (E), CD8⁺ T cells (F), and CD4⁺ T cells (G). Data represent the mean of n = 4 to 8 samples from two independent experiments. Data were analyzed by unpaired t test. ****, P < 0.0001; ***, P < 0.0005; **, P < 0.005.