FIG 7.

Flow cytometric quantitation of naive (T_N), effector memory (T_EM), and central memory (T_CM) CD8⁺ and CD4⁺ T cell subsets in the spleens of naive and γHV68-infected mice. WT and STAT1 R274W mice were inoculated intraperitoneally at age 5 to 6 weeks with 10⁶ PFU of γHV68 or PBS control (mock, day 0). Infected mice were euthanized and splenocytes harvested at days 0, 4, 8, and 14 for flow cytometric analysis of CD8⁺ and CD4⁺ naive (CD44^loCD62L^hi) T cells, effector memory (CD44^hiCD62L^lo) T cells, and central memory (CD44^hiCD62L^hi) T cells. (A and B) Percentage and numbers of naive (T_N), effector memory (T_EM), and central memory (T_CM) CD8⁺ (A) and CD4⁺ (B) T cell subsets at the indicated time points. Data represent the mean ±SEM of n = 4 to 8 mice per genotype at each time point. Data were analyzed by 2-way ANOVA. ****, P < 0.0001; ***, P < 0.0005; **, P < 0.005; *, P < 0.05.