FIG 1.

HBZ expression in Jurkat cells stimulates homotypic aggregation. (A) Western blot (WB) detection of HBZ expression in Jurkat clonal cell lines and HTLV-1-infected T-cell lines. HBZ was affinity purified from whole-cell extracts using the CH1-KIX domain of CBP fused to GST. The upper panel shows the membrane probed with an HBZ antibody. The lanes shown are from the same membrane/same scanned image. The lower panel shows the same membrane stained with Ponceau S. An asterisk denotes a nonspecific band. (B) Homotypic aggregation of the indicated T-cell lines. The cells indicated were plated at 5 × 10⁵ cells/ml, at which time cellular aggregates were completely disrupted. Cells were cultured for 2 h to allow aggregates to reform and then were photographed. (C) Homotypic aggregation of the indicated empty-vector and HBZ-expressing Jurkat clones. The cells indicated were plated at 1 × 10⁶ cells/ml, at which time cellular aggregates were completely disrupted. Cells were cultured for 3 h to allow aggregates to reform and then were photographed.