FIG 2.

Mg²⁺ induces homotypic aggregation of HBZ-expressing Jurkat clones. (A) Effects of divalent metal ion chelators on homotypic aggregation. The cells indicated were plated at 5 × 10⁵ cells/ml in the absence or present of the indicated chelators, at which time cellular aggregates were completely disrupted. Cells were cultured for 6 h to allow to aggregates to reform and were then photographed. (B) Effects of Mg²⁺ and Ca²⁺ on homotypic aggregation. The cells indicated were plated at 5 × 10⁵ cells/ml in HEPES-buffered Tyrode’s solution without Mg²⁺ or Ca²⁺ (unsupplemented) or containing 1 mM Mg²⁺ or Ca²⁺ as indicated. Cells were cultured for 6 h and then photographed.