FIG 6.

Among the ICAM family members, HBZ-mediated activation is restricted to ICAM-1 and does not depend on JunD. (A) ICAM1 mRNA levels in empty-vector and HBZ-expressing HeLa clones. The graph shows qRT-PCR data averaged from three independent experiments, with data normalized to values for the empty-vector clone (set to 1). Mutations in the activation domain, leucine zipper domain, and the start codon of HBZ are indicated as mutAD, mutZIP, and mutATG, respectively; wt, wild type. Error bars show standard deviations. Significance was determined by a two-tailed Student's t test (***, P < 0.001). Western blot analysis was performed using whole-cell extracts prepared from HeLa clones. Membranes were probed with an antibody against Myc to detect the C-terminal epitope tag on HBZ and actin. (B) JUND and ICAM1 mRNA levels in an HBZ wild-type-expressing HeLa clone following siRNA-mediated knockdown of JUND. The graph shows qRT-PCR data averaged from three independent experiments for ICAM1 and two experiments for JUND, with data normalized to values for the control siRNA (set to 1). (C) ICAM2, ICAM3, ICAM4, and ICAM5 mRNA levels in empty-vector and HBZ-expressing Jurkat clones. The graph shows qRT-PCR data averaged from three independent experiments, with data normalized to values for empty-vector clone C2 (set to 1). Error bars show standard deviations.