Skip to main content
. 2019 Sep 12;93(19):e00733-19. doi: 10.1128/JVI.00733-19

FIG 3.

FIG 3

Luciferase reporter replicon systems enable direct quantification of RHV replication. (A) Absolute FLuc activity per well after transfection of parental McA-RH7777 cells with bicistronic FEO replicons with and without dinucleotide optimization. (B) FLuc activity after transfection of McA-RH7777 cells as described for panel A but with comparison of single and combined substitutions. (C) Comparison of FLuc activity per well after transfection of McA-RH7777 cells with mono- or bicistronic versions of the FEO replicon. (D) Replication efficiency of expanded rat McA-RH7777 (red) and mouse Hepa1-6 (blue) FEO cell clones. FLuc activity per cell was measured 4 weeks after electroporation with RHV-adFEO-10 RNA and subsequent G418 selection. (E) Absolute FLuc activity per well in parental rat McA-RH7777 (red) and mouse Hepa1-6 (blue) cells, measured 4 h after electroporation with a replication-deficient FEO-GNN replicon. Different passage numbers are shown, and the background luminescence level is indicated by the dashed line. All data points in panels A to E represent means ± SD from triplicates.