KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-ChREBP | Novus Biologicals | NB400135 lots Q1-Q3 |
| Rabbit polyclonal anti-HCF-1 | Bethyl laboratories | A301-400A |
| Rabbit monoclonal anti-OGT | Cell Signaling | 24083 |
| Rabbit monoclonal anti-PHF2 | Cell Signaling | 3497S |
| Rabbit polyclonal anti-GCK | Santa Cruz | sc7908 |
| Rabbit monoclonal anti-BAD | Abcam | ab32445 |
| Rabbit polyclonal anti-phospho BAD (S155) | Cell Signaling | 9297 |
| Mouse monoclonal anti-Actin | Sigma-Aldrich | A5316 |
| Rabbit IgG Isotype Control | Invitrogen | 31235 |
| Mouse monoclonal anti FLAG M2- Peroxidase | Sigma-Aldrich | A8592 |
| Mouse Anti-rabbit IgG (Conformation Specific) | Cell Signaling | 5127S |
| Goat anti-rabbit IgG HRP | Jackson ImmunoResearch Laboratories | 111-035-003 |
| Goat anti-mouse IgG HRP | Jackson ImmunoResearch Laboratories | 115-035-003 |
| Bacterial and Virus Strains | ||
| BAD SD Adenovirus | Gimenez-Cassina et al, 2014 | PMID: 24506868 |
| BAD AAA Adenovirus | Gimenez-Cassina et al, 2014 | PMID: 24506868 |
| Gk shRNA Adenovirus | Gift of Dr. Christopher Newgard (Duke University) Bain et al., 2004 | PMID: 15331526 |
| Bad shRNA Adenovirus | Gimenez-Cassina et al, 2014 | PMID: 24506868 |
| Chrebp shRNA Adenovirus | Designed from sequence in Dentin et al, 2006 | PMID: 16873678 |
| Ctrl shRNA Adenovirus | Gimenez-Cassina et al, 2014 | PMID: 24506868 |
| FLAG-ChREBP Adenovirus | Gift from Dr. Donald Scott (Mount Sinai) (Metukuri et al, 2012) | PMID: 22586588 |
| Biological Samples | ||
| Human Liver Tissues Healthy and NASH Patients | Liver Tissue Cell Distribution System, Minneapolis, Minnesota | NIH Contract # HHSN276201200017C |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Phosphatase Inhibitor Cocktail 2 aqueous solution | Sigma-Aldrich | P5726 |
| Protein Phosphatase Inhibitor Cocktail 3 | Sigma-Aldrich | P0044 |
| Phosstop, Phosphatase Inhibitor | Sigma-Aldrich | 4906837001 |
| Complete™, EDTA-free Protease Inhibitor Cocktail | Sigma-Aldrich | 11873580001 |
| PuGNAC | Sigma-Aldrich | A7229 |
| Thiamet G | Sigma-Aldrich | SML 0244 |
| Sodium Orthovanadate | Sigma-Aldrich | S6508 |
| Western Lightning Plus-ECL | Thermo Fisher Scientific | 509049325 |
| SuperSignal West Femto | Thermo Fisher Scientific | PI34095 |
| NuPAGE LDS Sample Buffer (4X) | Life Technologies | NP0008 |
| FLAG Peptide | Sigma-Aldrich | F3290 |
| UDP-GlcNAC | Sigma-Aldrich | |
| N-Acetyl-D-glucosamine | Sigma-Aldrich | A8625-5G |
| Proteinase K | Life technologies | 25530015 |
| CHELEX 100 200-400 MB | Bio-Rad Laboratories | 1421253 |
| Formaldehyde (37% solution) | Santa Cruz | sc-203049 |
| Collagenase | Sigma-Aldrich | C5138 |
| Insulin | Sigma-Aldrich | I6634 |
| Dexamethasone | Sigma-Aldrich | D-2915 |
| Percoll | Sigma-Aldrich | P4937 |
| MG132 | Sigma-Aldrich | C2211 |
| BSA Fraction IV Fatty acid free | Fischer Scientific | NC9227912 |
| Glucosamine hydrochloride | Sigma-Aldrich | G4875 |
| Ammonium Formate | Sigma-Aldrich | 17843 |
| Acetonitrile | Sigma-Aldrich | AX0145-1 |
| HPLC grade water | Sigma-Aldrich | WX0008 |
| Ammonium Acetate | Sigma-Aldrich | 5330040050 |
| U14C labeled glucose | American Radiolabled Chemical | ARC-122 |
| Critical Commercial Assays | ||
| Dual luciferase reagent | Promega | E1910 |
| Targefect hepatocyte | Targeting systems | HEP-01 |
| Trizol | Life Technologies | 15596026 |
| Syber Green Master Mix | Life Technologies | |
| Superscript III 1st Strand Synthesis | Life Technologies | 18080051 |
| Lipofectamine RNAiMax | Life Technologies | 13778150 |
| Q5 Site-Directed Mutagenesis Kit | New England Biolabs | E0554S |
| Gateway™ LR Clonase™ II Enzyme mix | Thermo Fischer Scientific | 11791100 |
| TnT® Coupled Wheat Germ Extract System | Promega | L4130 |
| Deposited Data | ||
| Unprocessed gel images presented in this manuscript | http://dx.doi.org/10.17632/by6b3tn4g2.1 | |
| Experimental Models: Cell Lines | ||
| HEK293T Cells | ATCC | CRL-3216 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6: WT | ||
| C57BL/6-KO (BAD) | Gimenez-cassina et al, 2014 | PMID: 24506868 |
| GKlox/lox | Gift from Dr. Mark Magnuson (Vanderbilt university): Postic et al, 1999 | PMID: 9867845 |
| C57BL/6-alb-cre mice | Jackson Laboratory | 003574 |
| Oligonucleotides | ||
| Ctrl siRNA: siGenome Non-targeting siRNA #1 | Dharmacon | D-001210 |
| Hcf1 siRNA-1 sense sequence: GGA GCU UAU AGU GGU GUU U | Dharmacon | N/A |
| Hcf1 siRNA-2 sense sequence: AGA ACA ACA UUC CGA GGU A | Dharmacon | N/A |
| Primers for qPCR: See Table S1 for sequence | IDT | N/A |
| Recombinant DNA | ||
| L-Pk ChORE firefly luciferase reporter | Gift from Dr. Howard Towle: Lou D-Q et al, 1999 | PMID: 10497199 |
| Acc ChORE firefly luciferase reporter | Gift from Dr. Howard Towle: O’Callaghan BL et al, 2001 | PMID: 11340083 |
| pCMV-Green Renilla Luciferase control plasmid: | Thermo Fisher Scientific | PI16153 |
| pCMV10 3X FLAG-ChREBP | Gift from Dr. Mark Herman (Duke University) | N/A |
| pCMV-SPORT6 HCF-1 Plasmid | Harvard PlasmID | MmCD00320375 |
| His-OGT WT | Janetzko et al, 2016 | PMID: 27618188 |
| His-OGT D554N | Janetzko et al, 2016 | PMID: 27618188 |
| OGT WT | This Paper | N/A |
| OGT D554N | This Paper | N/A |
| PCDNA3.1 FLAG-PGC1α | Gift from Dr. Pere Puigserver: Fan et al, 2004 | PMID: 14744933 |
| pCDNA3.1 | Invitrogen | V790-20 |
| Software and Algorithms | ||
| ImageJ | https://imagej.nih.gov/ij/ | N/A |
| Prism | Graph Pad | N/A |
| SDS 2.4 (for Q-PCR) | Applied Biosystems | N/A |
| MassHunter Quant | Agilent Technologies | N/A |
| Sequest | Thermo Fischer Scientific | N/A |
| Other | ||
| Serum | Gemini | 100-106 lot A16E00E |
| High Carbohydrate Diet (70% sucrose) | Envigo | TD.150694 |
| WGA Beads | Vector Laboratories | AL-1023 |
| Anti-FLAG® M2 Magnetic Beads | Sigma-Aldrich | M8823 |
| Protein G Agarose | Sigma-Aldrich Aldrich | 11719416001 |
| DynaBeads Protein A | Thermo Fisher Scientific | 10001D |
| Amicon Ultra Centrifugal Filters | Millipore | UFC500324 |