Fig. 5. Basal and stimulated K-Cl cotransport activity in WT and SAD (B) red cells.

K-Cl cotransport activity of WT and SAD red cells was measured in isotonic medium (ISO), hypotonic medium (Hypo), isotonic medium containing 1 μM staurosporine (Stauro), and isotonic medium with added 500 mM urea (Urea). Values are means ± SEM for (n) independent experiments with blood pooled from 2–4 mice of indicated genotype. ISO WT red cells (n=5) included WT^C57 (n=2), WT^Kcnn4 (n=2) and WT^SAD (n=1). WT red cells in stimulated conditions were (for HYPO, n=10) 1 WT^C57 + 9 WT^Kcnn4; (for Stauro, n=8) 1 WT^C57 + 7 WT^Kcnn4; and (for Urea, n=9) 1 WT^C57, 1 WT^SAD, and 7 WT^Kcnn4. WT and SAD samples were compared for each condition by Mann-Whitney non-paired test.