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Cross-linking experiments with BS3 to identify the interaction between 40 μM IscS and 40 μM FXN wild-type (2) or mutated: p.D104G (3), p.A107V (4), p.F109L (5), p.Y123S (6), p.S161I (7), p.S181F (8), p.S202F (9). The first lane on the left is the control carried out using isolated IscS (1). The new species at ca. 58 kDa corresponds to the complex FXN-IscS.
