Table 2.

Folding kinetics data for FXN wild type and variants calculated from chevron plot fitting.

	akf (s−1)	amf (kcal/ mol•M)	aku (s−1)	amu (kcal/ mol•M)	am’ (kcal/ mol•M)	bΔGN-D (kcal/mol)
Wild type	78.5 ± 5.8	0.64 ± 0.02	7.8·10−5 ± 0.8·10−5	1.22 ± 0.06	0.06± 0.01	8.0 ± 0.8
p.D104G	84.5 ± 5.9	0.64 ± 0.02	3.2·10−5 ± 0.3·10−5	1.22 ± 0.06	0.06± 0.01	8.6 ± 0.9
p.A107V	70.0 ± 5.4	0.64 ± 0.02	1.6·10−5 ± 0.2·10−5	1.22 ± 0.06	0.06± 0.01	7.5 ± 0.8
p.F109L	57.7 ± 3.6	0.64 ± 0.02	1.1·10−5 ± 0.1·10−5	1.22 ± 0.06	0.06± 0.01	5.0 ± 0.5
p.Y123S	43.8 ± 2.9	0.64 ± 0.02	3.4·10−5 ± 0.3·10−5	1.22 ± 0.06	0.06± 0.01	4.1 ± 0.4
p.S161I	21.7 ± 1.4	0.64 ± 0.02	1.9·10−5 ± 0.2·10−5	1.22 ± 0.06	0.06± 0.01	5.4 ± 0.5
p.S181F	32.7 ± 2.1	0.64 ± 0.02	3.0·10−5 ± 0.3·10−5	1.22 ± 0.06	0.06± 0.01	5.4 ± 0.5
p.S202F	71.4 ± 4.9	0.64 ± 0.02	9.4·10−5 ± 0.9·10−5	1.22 ± 0.06	0.06± 0.01	7.9 ± 0.8

^ak_f, m_f, m_f, k_u, m_u and m’ were calculated from Eq (5).

^bΔG_N-D values were calculated from Eq (6)