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Identification of glycosites and glycopeptides from bovine fetuin with and without ethanol-EDA derivatization. (a) Three glycosites N[99]CSV, N[156]DSR, and N[176]GSY, containing 93, 61, 99 unique sialoglycopeptides, respectively (in triplicates), on native fetuin after HILIC enrichment. (b) Three glycosites containing 127, 102, and 278 sialoglycopeptides after ethanol-EDA modification. Sialic acid linkage is differentially determined by IntactGIG-HILIC
