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. 2018 Apr 12;29(6):1273–1283. doi: 10.1007/s13361-018-1931-0

Table 4.

Identification of Sialoglycopeptides from Human Serum Using IntactGIG Method. α2,6-Linked Sialoglycopeptides Are Abundantly Present in Human Serum

Glycoprotein Uniprot ID Glycosite Sequence Mass tag on glycan Glycan
Alpha 1-acid glycoprotein AAB33887 56 R.NeEYnK.S HexNAc(3)Hex(4) H4N3
AAB33887 103 R.EnGTISR.Y HexNAc(2) N2
AAB33887 33 M.ALSWVLTVLSLLPLLEAQIPLCANLVPVPITnATLDQITGK.W HexNAc(2) N2
Alpha-2-macroglobulin P01023 70 R.GnR.S HexNAc(3)Hex(4) H4N3
HexNAc(4)Hex(5)NeuAc(2) 56.062582 S2(2,6)H5N4
HexNAc(4)Hex(5)Fuc(1)NeuAc(2)Na(2) 56.06258067 S2(2,6)H5N4F1 [2Na]
HexNAc(4)Hex(5)NeuAc(1) 28.031275 S1H5N4
HexNAc(4)Hex(5)Fuc(1)NeuAc(2) 56.062574 S2(2,6)H5N4F1
HexNAc(2)Hex(7) H7N2
HexNAc(4)Hex(4) H4N4
Amyloid-like protein 1 P51693 461 R.FQVHTHLQVIEERVnQSLGLLDQNPHLAQELRPQIQELLHSEHLGPSELEAPAPGGSSEDK.G HexNAc(4)Hex(4)NeuAc(1)Na(1) 42.05816434 S1(2,3)H4N4[Na]
Cadherin-2 P19022 338 R.IVSQAPSTPSPNMFTINnETGdIITVAAGLdREK.V HexNAc(2)Fuc(1) H2F1
Endoplasmin P14625 502 K.LGVIEDHSnRTR.L HexNAc(4)Hex(5)NeuAc(2) 84.116346 S2(2,3)H5N4
Haptoglobin 4WJG 150 K.VVLHPnYSQVDIGLIK.L HexNAc(4)Hex(5)NeuAc(2) 56.062582 S2(2,6)H5N4
HexNAc(4)Hex(5)NeuAc(2)Na(2) 70.08947067 S1(2,6)S1(2,3)H5N4[2Na]
Hemopexin P02790 453 K.ALPQPQnVTSLLGCTH.- HexNAc(4)Hex(5)NeuAc(2) 56.062582 S2(2,6)H5N4
187 R.SWPAVGnCSSALR.W HexNAc(4)Hex(5)NeuAc(2) 56.062582 S2(2,6)H5N4
Integrin alpha-2 P17301 1074 K.GEYFVnVTTR.I HexNAc(2)Hex(3) H3N2
Integrin beta-1 P05556 94 K.NKnVTNR.S HexNAc(4)Hex(5)Fuc(1)NeuAc(1) 28.031267 S1(2,6)H5N4F1
Titin Q8WZ42 21153 R.AnK.T HexNAc(4)Hex(5)NeuAc(2) 56.062582 S2(2,6)H5N4
R.AnK.T HexNAc(4)Hex(5)Fuc(1)NeuAc(2)Na(2) 70.08946267 S1(2,6)S1(2,3)H5N4F1[2Na]

N, hexNAc; H, hexose; F, fucose; S, NeuAc