Figure 2.

NCoR1 Depletion Enhances Differentiation of Naive CD4⁺ Th Cells into Tregs

(A) Experimental outline used to assess the effects of NCoR1 KD on T cell polarization ex vivo.

(B) Representative histograms showing the MFI shifts observed for proliferation of T helper cells co-cultured with unstimulated or CpG-activated NCoR1 KD and control cDC1 DCs (n = 3).

(C) Histogram plots demonstrating the representative MFI shifts observed for FoxP3, Tbet, IFNγ, and GATA3 in helper T cells co-cultured with NCoR1 KD and control cDC1 DCs for 5 days (n = 5).

(D) Scatterplots from flow cytometric analysis showing the percentage positive T helper cells expressing CD25⁺FoxP3⁺, Tbet, IFNγ, and GATA3 after 5 days of co-culture with activated NCoR1 KD and control DCs (n = 5).

p values are calculated using two-sample unpaired Student's t test; error bars represent SEM. *p ≤ 0.05, ***p ≤ 0.001.