Figure 3.

Conventional DCs from NCoR1^DC−/- Mice Show Increased Tolerogenic Behavior upon Activation

Splenocytes from NCoR1^DC−/- and WT mice were treated with and without CpG for 6 h and the impact of NCoR1 ablation on different DC subsets was analyzed by flow cytometry.

(A) Scatterplots depicting the percentage positive cells for IL-10, IL-27, IDO1, and IL-6 in primary cDC1 DCs from NCoR1^DC−/- and WT mice. Corresponding histogram plots depict the MFI shifts (n = 6).

(B) Scatterplots demonstrating the percentage positive cells for IL-10, IL-27, IDO1, and IL-6 in primary cDC2 DCs from NCoR1^DC−/- and WT mice (n = 6).

(C) and (D) Scatterplot showing the percentage of CD80-positive cells in primary cDC1 and cDC2 DCs respectively from NCoR1^DC−/- and WT mice before and after 6 h CpG activation. Representative histograms depict the MFI shift.

(E) Experimental outline depicting the method employed to identify the in vivo impact of NCoR1 depletion on CD4⁺ T cell polarization in NCoR1^DC−/- and WT mice.

(F) Dot plots showing the percentage of CD4⁺CD44⁺FoxP3⁺-expressing effector T cells from the draining inguinal lymph nodes of NCoR1^DC−/- and WT mice vaccinated with CpG and OVA for 1 month (n = 15).

p values are calculated using two-tailed unpaired Student's t test; error bars represent SEM. *p ≤ 0.05, **p ≤ 0.01. See also Figures S3 and S4.