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. 2019 Sep 13;9:13259. doi: 10.1038/s41598-019-49853-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FAK inhibition led to decreased survival and proliferation in COA3 and COA6 sorted cell populations. COA3 and COA6 cells were sorted for CD133 using a magnetic cell sorting system. (A) COA3 CD133-enriched or CD133-depleted cells (1.5 × 10⁴) were treated with increasing concentrations of PF (0, 5, 20 μM) or Y15 (0, 5, 10 μM) for 5 days and cell survival was measured using alamarBlue® assay. There was a significant decrease in cell survival in both populations following treatment with PF and Y15. (B) COA6 CD133-enriched or CD133-depleted cells (1.5 × 10⁴) were treated in the same fashion, and there was a significant decrease in cell survival following treatment with PF and Y15 in both populations. (C) COA3 CD133-enriched or CD133-depleted cells (5 × 10³) were treated with increasing concentrations of PF or Y15 for 5 days and cell proliferation was measured using CellTiter96® assay. Proliferation was significantly decreased following treatment with PF and Y15 in both cell populations. (D) COA6 CD133-enriched or CD133-depleted cells (5 × 10³) were treated in the same fashion, and proliferation was significantly decreased following treatment with PF and Y15 in both cell populations. Data represent at least three biologic replicates and are reported as mean ± SEM.