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. 2019 Sep 13;10:4175. doi: 10.1038/s41467-019-11977-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Function of periplasmic domains of LptF and LptG. The structure of the β-jellyroll-like periplasmic domains of LptF and LptG is similar to that of LptC. a Cartoon representation of LptF. Hydrophobic β-jellyroll-like core residues F149, P139, Y230 and R212 are shown in stick. b Functional assays of LptF residues. Residues D129 and E265 are at the neck of the cavity as a control. Mutants R212E/Y230E, P139D/F149D, Y230E and F149D are lethal, while mutants D129A/E265A, R212E and P139D do not have any impact on cell growth. c Protein expression level of the mutants was detected by western blotting. Protein expression levels of other mutants are higher than that of P139D. Source data are provided as a Source Data file (7c). d Cartoon representation of LptG. Hydrophobic β-jellyroll-like core residues I163, W204, L206 and V209 are shown in stick. e Functional assays of LptG residues. K62 is located at the cavity as a control, and the mutant K62E grows the same as the wild type. Mutants W204D, I163D, and L206D are lethal, and mutant V209D reduces the cell growth. f Protein expression level of mutants was detected by western blotting. Protein expression level of other mutants is higher than that of mutant K62E except mutant I163D. The lethality of mutant I163D might cause by the lower protein expression. Source data are provided as a Source Data file (7f)